Synergistic In Vitro Antimicrobial Activity of Methanolic Extract of Pomegranate Peel and Black Seed

Cholleti  Raghunath; Marya  Habeeba; Afreen  Muskan; Fateh  Ullah; Naved  Khan

doi:10.52816/JDVAT.2021.1303

Authors

Cholleti Raghunath Research Scholar, Department of Pharmacognosy, Global College of Pharmacy, Jawaharlal Nehru Technological University, Ranga Reddy District, Telangana, India – 501 504.
Marya Habeeba Research scholar, Department of Pharmacognosy, Global College of Pharmacy, Jawaharlal Nehru Technological University, Ranga Reddy District, Telangana, India – 501 504.
Afreen Muskan Research Scholar, Department of Pharmacognosy, Global College of Pharmacy, Jawaharlal Nehru Technological University, Ranga Reddy District, Telangana, India – 501 504
Fateh Ullah Research Scholar, Department of Pharmacognosy, Global College of Pharmacy, Jawaharlal Nehru Technological University, Ranga Reddy District, Telangana, India – 501 504
Naved Khan Assistant Professor, Department of Pharmacognosy, Global College of Pharmacy, Jawaharlal Nehru Technological University, Ranga Reddy District, Telangana, India – 501 504.

DOI:

https://doi.org/10.52816/JDVAT.2021.1303

Keywords:

Antimicrobial activity, pomegranate, black seed, synergism, disc diffusion, agar diffusion

Abstract

The disc diffusion and agar well diffusion methods were used to investigate the synergistic in vitro antibacterial activity of methanolic extracts of pomegranate peel and black seed. Extracts of pomegranate peel and black seed were produced in methanol for this objective and evaluated in vitro against Bacillus cereus, Staphylococcus aureus, Escherichia coli, Salmonella typhi, and Pseudomonas aeruginosa bacterial strains. The results were then compared to the standard drug gentamycin (50 μg). The synergistic in vitro antibacterial activity of pomegranate peel and black seed methanolic extracts revealed varying levels of antimicrobial activity against all test microorganisms.